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Background  Key mechanisms of cell division and its regulation are well understood in model bacteria such as  Escherichia coli  and  Bacillus subtilis.  In contrast, current knowledge on the regulation of cell division in  Actinobacteria  is rather limited. FtsZ is one of the key players in this process, but nothing is known about its transcriptional regulation in  Corynebacterium glutamicum,  a model organism of the  Corynebacteriales .    Results  In this study, we used DNA affinity chromatography to search for transcriptional regulators of  ftsZ  in  C. glutamicum  and identified the Cg1631 protein as candidate, which was named FtsR. Both deletion and overexpression of  ftsR  caused growth defects and an altered cell morphology. Plasmid-based expression of native  ftsR  or of homologs of the pathogenic relatives  Corynebacterium diphtheriae  and  Mycobacterium tuberculosis  in the Δ ftsR  mutant could at least partially reverse the mutant phenotype. Absence of  ftsR  caused decreased expression of  ftsZ , in line with an activator function of FtsR. In vivo crosslinking followed by affinity purification of FtsR and next generation sequencing of the enriched DNA fragments confirmed the  ftsZ  promoter as in vivo binding site of FtsR and revealed additional potential target genes and a DNA-binding motif. Analysis of strains expressing  ftsZ  under control of the gluconate-inducible  gntK  promoter revealed that the phenotype of the Δ ftsR  mutant is not solely caused by reduced  ftsZ  expression, but involves further targets.    Conclusions  In this study, we identified and characterized FtsR as the first transcriptional regulator of FtsZ described for  C. glutamicum . Both the absence and the overproduction of FtsR had severe effects on growth and cell morphology, underlining the importance of this regulatory protein. FtsR and its DNA-binding site in the promoter region of  ftsZ  are highly conserved in  Actinobacteria , which suggests that this regulatory mechanism is also relevant for the control of cell division in related  Actinobacteria .    Electronic supplementary material  The online version of this article (10.1186/s12866-019-1553-0) contains supplementary material, which is available to authorized users.

bmc microbiology

The conserved actinobacterial transcriptional regulator FtsR controls expression of ftsZ and further target genes and influences growth and cell division in Corynebacterium glutamicum