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Background  In natural environments, bacteria always live in communities with others where their physiological characteristics are influenced by each other. Bacteria can communicate with one another by using autoinducers. The current knowledge on the effect of quenching bacteria on others is limited to assess the impact of quorum-quenching bacterium  Bacillus  sp. QSI-1 on proteins pattern and virulence factors production of  Aeromonas hydrophila  YJ-1 .  Proteomic analysis was performed to find out protein changes and virulence factors, after 24 h co-culture.    Results  Results showed that several proteins of  A. hydrophila  YJ-1 were altered, seventy-two differentially expressed protein spots were excised from 2-DE gels and analyzed by MALDI-TOF/TOF MS, resulting in 63 individual proteins being clearly identified from 70 spots. Among these proteins, 50 were divided into 22 classes and mapped onto 18 biological pathways. Mixed-culture growth with  Bacillus  sp. QSI-1 resulted in an increase of  A. hydrophilia  proteins involved in RNA polymerase activity, biosynthesis of secondary metabolites, flagellar assembly, and two-component systems. In contrast, mixed culture resulted in a decreased level of proteins involved in thiamine metabolism; valine, leucine and isoleucine biosynthesis; pantothenate and CoA biosynthesis. In addition, the two extracellular virulence factors, proteases and hemolysin, were significantly reduced when  A. hydrophila  was co-cultured with QSI-1, while only lipase activity was observed to increase.    Conclusions  The information gathered from our experiment showed that  Bacillus  sp. QSI-1 has a major impact on the expression of proteins, including virulence factors of  A. hydrophila .    Electronic supplementary material  The online version of this article (10.1186/s12866-019-1515-6) contains supplementary material, which is available to authorized users.

Effect of quorum-quenching bacterium Bacillus sp. QSI-1 on protein profiles and extracellular enzymatic activities of Aeromonas hydrophila YJ-1