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Background   Acinetobacter baumannii  is a cosmopolitan bacterium that is frequently reported from hospitalized patients, especially those patients who admitted in the intensive care unit. Recently, multiplex real-time PCR has been introduced for rapid detection of the resistance genes in clinical isolates of bacteria. The current study aimed to develop and evaluate multiplex real-time PCR to detect common resistance genes among clinical isolates of  A. baumannii .    Results  Multiplex real-time PCR based on melting curve analysis showed different T m  corresponding to the amplified fragment consisted of 83.5 °C, 93.3 °C and 89.3 °C for  bla IMP,  bla VIM and  bla NDM, respectively. Results of multiplex real-time PCR showed that the prevalence of  bla IMP,  bla VIM and  bla NDM among the clinical isolates of  A. baumannii  were 5/128(3.9%), 9/128(7.03%) and 0/128(0%), respectively. Multiplex real-time PCR was able to simultaneously identify the resistance genes, while showed 100% concordance with the results of conventional PCR.    Conclusions  The current study showed that  bla VIM, was the most prevalent MBL gene among the clinical isolates of  A. baumannii  while no amplification of  bla NDM was seen. Multiplex real-time PCR can be sensitive and reliable technique for rapid detection of resistance genes in clinical isolates.

bmc microbiology

Rapid detection and molecular survey of blaVIM, blaIMP and blaNDM genes among clinical isolates of Acinetobacter baumannii using new multiplex real-time PCR and melting curve analysis