English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Concern exists that frequent use of topically-applied fusidic acid (FA) and chlorhexidine (CHX) for canine pyoderma is driving clinically relevant resistance, despite rare description of FA and CHX genetic resistance determinants in canine-derived staphylococci. This study aimed to determine minimum inhibitory concentrations (MICs) and investigate presence of putative resistance determinants for FA and CHX in canine-derived methicillin-resistant (MR) and -susceptible (MS) staphylococci. Plasmid-mediated resistance genes ( fusB, fusC, fusD, qacA/B, smr;  PCR) and MICs (agar dilution) of FA and CHX were investigated in 578 staphylococci (50 MR  S. aureus  [SA], 50 MSSA, 259 MR  S. pseudintermedius  [SP], 219 MSSP) from Finland, U.S.A., North (NUK) and South-East U.K. (SEUK) and Germany. In all isolates with FA MIC ≥64 mg/L ( n  = 27)  fusA  and  fusE  were amplified and sequenced.    Results  FA resistance determinants ( fusA  mutations  n  = 24,  fusB  n = 2,  fusC n  = 36) were found in isolates from all countries bar U.S.A. and correlated with higher MICs (≥1 mg/L), although 4 SP isolates had MICs of 0.06 mg/L despite carrying  fusC . CHX MICs did not correlate with  qacA/B  (n = 2) and  smr  ( n  = 5), which were found in SEUK SA, and SP from NUK and U.S.A.    Conclusions  Increased FA MICs were frequently associated with  fusA  mutations and  fusC,  and this is the first account of  fusB  in SP. Despite novel description of  qacA/B  in SP, gene presence did not correlate with CHX MIC. Selection pressure from clinical use might increase prevalence of these genetic determinants, but clinical significance remains uncertain in relation to high skin concentrations achieved by topical therapy.

Genetic resistance determinants to fusidic acid and chlorhexidine in variably susceptible staphylococci from dogs