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Background   Acinetobacter baumannii  is an opportunistic pathogen that causes serious nosocomial infection in intensive care units. In particular, carbapenem-resistant  A. baumannii  (CRAB) strains have been increasing in the past decade, and they have caused major medical problems worldwide. In this study, a novel  A. baumannii  lytic phage, the YMC 13/03/R2096 ABA BP (phage Βϕ-R2096), which specifically causes the lysis of CRAB strains, was characterized in detail in vitro and in silico, and the in vivo effectiveness of phage therapy was evaluated using  Galleria mellonella  and a mouse model of acute pneumonia.    Results  The  A. baumannii  phage Βϕ-R2096 was isolated from sewage water using CRAB clinical strains selected from patients at a university hospital in South Korea. The complete genome of the phage Βϕ-R2096, which belongs to the  Myoviridae  family, was analyzed. Phage Βϕ-R2096 inhibited bacterial growth in a dose-dependent manner and exhibited high bacteriolytic activity at MOI = 10. In the evaluation of its therapeutic potential against CRAB clinical isolates using two in vivo models, phage Βϕ-R2096 increased the survival rates of both  G. mellonella  larvae (from 0 to 50% at 24 h) and mice (from 30% with MOI = 0.1 to 100% with MOI = 10 for 12 days) in post-infection of CRAB. In particular, phage Βϕ-R2096 strongly ameliorated histologic damage to infected lungs, with bacterial clearance in the lungs observed on day 3 postinfection in the mouse acute pneumonia model. Moreover, in vivo studies revealed no mortality or serious side effects in phage-treated groups.    Conclusion  The results of this study strongly suggest that phage Βϕ-R2096, a novel  A. baumannii  lytic phage, could be an alternative antibacterial agent to control CRAB infections. This study is the first report to compare in vivo evaluations ( G. mellonella  larvae and a mouse acute pneumonia model) of the therapeutic efficacy of a phage against CRAB infections.    Electronic supplementary material  The online version of this article (10.1186/s12866-019-1443-5) contains supplementary material, which is available to authorized users.

bmc microbiology

Efficacy of bacteriophage treatment against carbapenem-resistant Acinetobacter baumannii in Galleria mellonella larvae and a mouse model of acute pneumonia