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Background  Extended-spectrum cephalosporin resistance (ESC-R) in  Escherichia coli  and  Klebsiella pneumoniae  is a healthcare threat; high gastrointestinal carriage rates are reported from South-east Asia. Colonisation prevalence data in Cambodia are lacking. The aim of this study was to determine gastrointestinal colonisation prevalence of ESC-resistant  E. coli  (ESC-R-EC) and  K. pneumoniae  (ESC-R-KP) in Cambodian children/adolescents and associated socio-demographic risk factors; and to characterise relevant resistance genes, their genetic contexts, and the genetic relatedness of ESC-R strains using whole genome sequencing (WGS).    Results  Faeces and questionnaire data were obtained from individuals < 16 years in north-western Cambodia, 2012. WGS of cultured ESC-R-EC/KP was performed (Illumina). Maximum likelihood phylogenies were used to characterise relatedness of isolates; ESC-R-associated resistance genes and their genetic contexts were identified from de novo assemblies using BLASTn and automated/manual annotation. 82/148 (55%) of children/adolescents were ESC-R-EC/KP colonised; 12/148 (8%) were co-colonised with both species. Independent risk factors for colonisation were hospitalisation (OR: 3.12, 95% CI [1.52–6.38]) and intestinal parasites (OR: 3.11 [1.29–7.51]); school attendance conferred decreased risk (OR: 0.44 [0.21–0.92]. ESC-R strains were diverse; the commonest ESC-R mechanisms were  bla  CTX-M  1 and 9 sub-family variants. Structures flanking these genes were highly variable, and for  bla  CTX-M-15, − 55 and − 27  frequently involved IS 26 . Chromosomal  bla  CTX-M  integration was common in  E. coli .    Conclusions  Gastrointestinal ESC-R-EC/KP colonisation is widespread in Cambodian children/adolescents; hospital admission and intestinal parasites are independent risk factors. The genetic contexts of  bla  CTX-M  are highly mosaic, consistent with rapid horizontal exchange. Chromosomal integration of  bla  CTX-M  may result in stable propagation in these community-associated pathogens.    Electronic supplementary material  The online version of this article (10.1186/s12866-019-1431-9) contains supplementary material, which is available to authorized users.

bmc microbiology

Epidemiology of paediatric gastrointestinal colonisation by extended spectrum cephalosporin-resistant Escherichia coli and Klebsiella pneumoniae isolates in north-west Cambodia