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Background   Streptococcus pluranimalium  is a new member of the  Streptococcus  genus isolated from multiple different animal hosts. It has been identified as a pathogen associated with subclinical mastitis, valvular endocarditis and septicaemia in animals. Moreover, this bacterium has emerged as a new pathogen for human infective endocarditis and brain abscess. However, the patho-biological properties of  S. pluranimalium  remain virtually unknown. The aim of this study was to determine the complete genome sequence of  S. pluranimalium  strain TH11417 isolated from a cattle with mastitis, and to characterize its antimicrobial resistance, virulence, and carbon catabolism.    Results  The genome of  S. pluranimalium  TH11417, determined by single-molecule real-time (SMRT) sequencing, consists of 2,065,522 base pair (bp) with a G + C content of 38.65%, 2,007 predicted coding sequence (CDS), 58 transfer RNA (tRNA) genes and five ribosome RNA (rRNA) operons. It contains a novel IS Spl1  element (a memeber of the IS 3  family) and a Ф11417.1 prophage that carries the  mef (A),  msr (D) and  lnu (C) genes. Consistently, our antimicrobial susceptibility test confirmed that  S. pluranimalium  TH11417 was resistant to erythromycin and lincomycin. However, this strain did not show virulence in murine pneumonia (intranasal inoculation, 10 7  colony forming unit – CFU) and sepsis (intraperitoneal inoculation, 10 7  CFU) models. Additionally, this strain is able to grow with glucose, lactose or galactose as the sole carbon source, and possesses a lactose-specific phosphoenolpyruvate-dependent phosphotransferase system (PTS).    Conclusions  We reported the first whole genome sequence of  S. pluranimalium  isolated from a cattle with mastitis. It harbors a prophage carrying the  mef (A),  msr (D) and  lnu (C) genes, and is avirulent in the murine infection model.

Characterization of Streptococcus pluranimalium from a cattle with mastitis by whole genome sequencing and functional validation