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Background  Free-living amoeba (FLA) are wide-spread protozoa that are found in different environmental sources including water, soil, dust, hospital units and ventilation areas. These amoebas can act as opportunistic or non-opportunistic pathogens. Among FLAs, some genera such as  Acanthamoeba  are important because of their potential pathogenic ability in humans .  The purpose of this study is to identify of  Acanthamoeba  isolated from pond water of parks in a tropical and subtropical region in the Middle East, and its relation with physicochemical parameters.From August to December 2015, 90 samples were collected from pond water of parks of 13 regions of Mashhad City. Physicochemical parameters were measured in situ. After filtering, the samples were cultured on Bacto-agar enriched with  Escherichia coli . PCR analysis was conducted on the culture-positive samples, and then the PCR products were sequenced. Statistical analysis was performed by SPSS software and Fisher’s Exact and Mann-Whitney test.    Results  Among the samples that were examined, 19 samples (21.1%) were positive for  Acanthamoeba . The sequencing revealed that  Acanthamoeba  isolates belonged to T4 genotype of  Acanthamoeba . There was no significant relationship between physicochemical parameters and the presence of  Acanthamoeba .    Conclusion  The prevalence of  Acanthamoeba  in pond water of parks was relatively high, but there was no significant relationship between physicochemical parameters and the presence of  Acanthamoeba .

bmc microbiology

Isolation and identification of Acanthamoeba from pond water of parks in a tropical and subtropical region in the Middle East, and its relation with physicochemical parameters