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Background  Since recognizing the interaction between  Brucella  and host cells is crucial to the elucidation of the infectious process,  Brucella  researches have prioritized the investigation of genes related to pathogenicity. To demonstrate the roles of  Brucella  genes, RAW 264.7 cells were infected with the  Brucella abortus  wild-type and mutant strains (generated using transposon mutagenesis), after which the different transcriptional responses of the infected cells were determined using microarray.    Results  Following infection, enhanced strategies for intracellular survival, such as down-regulation of genes associated with cytokine responses and apoptosis, were observed in RAW 264.7 cells infected with C3 mutant strain when compared to the transcriptional responses of wild-type infected cells. Using sequence analysis, we determined the mutation site of a C3 mutant strain as the ATP-binding cassette transporter permease (BruAb2_1031). These results were evidenced by an increased level of intracellular survival of the C3 mutant strain.    Conclusions  Characteristics of each mutant strain including bacterial growth rate, abilities to induce cytokine production in macrophages after infection, internalization, and levels of intracellular survival and replication, were investigated by performing RAW 264.7 cell infection experiments. Our results indicate that the BruAb2_1031 gene might be closely related with intracellular survival of  B. abortus  in RAW 264.7 cells.    Electronic supplementary material  The online version of this article (10.1186/s12866-018-1223-7) contains supplementary material, which is available to authorized users.

Global gene-expression profiles of intracellular survival of the BruAb2_1031 gene mutated Brucella abortus in professional phagocytes, RAW 264.7 cells