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Background  The bacterial spirochete  Borrelia burgdorferi  is the causative agent of the most commonly reported arthropod-borne illness in the United States, Lyme disease. A family of proteins containing von Willebrand Factor A (VWFA) domains adjacent to a MoxR AAA+ ATPase have been found to be highly conserved in the genus  Borrelia . Previously, a VWFA domain containing protein of  B. burgdorferi,  BB0172, was determined to be an outer membrane protein capable of binding integrin α3β1. In this study, the characterization of a new VWFA domain containing membrane protein, BB0173, is evaluated in order to define the location and topology of this multi-spanning membrane protein. In addition, functional predictions are made.    Results  Our results show that BB0173, in contrast to BB0172, is an inner membrane protein, in which the VWFA domain is exposed to the periplasmic space. Further, BB0173 was predicted to have an aerotolerance regulator domain, and expression of BB0173 and the surrounding genes was evaluated under aerobic and microaerophilic conditions, revealing that these genes are downregulated under aerobic conditions. Since the VWFA domain containing proteins of  B. burgdorferi  are highly conserved, they are likely required for survival of the pathogen through sensing diverse environmental oxygen conditions.    Conclusions  Presently, the complex mechanisms that  B. burgdorferi  uses to detect and respond to environmental changes are not completely understood. However, studying the mechanisms that allow  B. burgdorferi  to survive in the highly disparate environments of the tick vector and mammalian host could allow for the development of novel methods of preventing acquisition, survival, or transmission of the spirochete. In this regard, a putative membrane protein, BB0173, was characterized. BB0173 was found to be highly conserved across pathogenic  Borrelia,  and additionally contains several truly transmembrane domains, and a  Bacteroides  aerotolerance-like domain. The presence of these functional domains and the highly conserved nature of this protein, strongly suggests a required function of BB0173 in the survival of  B. burgdorferi.

Characterization of the inner membrane protein BB0173 from Borrelia burgdorferi