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Background  Acetyl-CoA-derived chemicals are suitable for multiple applications in many industries. The bio-production of these chemicals has become imperative owing to the economic and environmental problems. However, acetate overflow is the major drawback for acetyl-CoA-derived chemicals production. Approaches for overcoming acetate overflow may be beneficial for the production of acetyl-CoA-derived chemicals.    Results  In this study, a transcriptional regulator  iclR  was knocked out in  E.coli  BL21(DE3) to overcome acetate overflow and improve the chemicals production. Two important acetyl-CoA-derived chemicals, phloroglucinol (PG) and 3-hydroxypropionate (3HP) were used to evaluate it. It is revealed that knockout of  iclR  significantly increased expressions of  aceBAK  operon. The cell yields and glucose utilization efficiencies were higher than those of control strains. The acetate concentrations were decreased by more than 50% and the productions of PG and 3HP were increased more than twice in  iclR  mutants. The effects of  iclR  knockout on cell physiology, cell metabolism and production of acetyl-CoA-derived chemicals were similar to those of  arcA  knockout in our previous study. However, the  arcA-iclR  double mutants couldn’t gain higher productions of PG and 3HP. The mechanisms are unclear and needed to be resolved in future.    Conclusions  Knockout of  iclR  significantly increased gene expression of  aceBAK  operon and concomitantly activated glyoxylate pathway. This genetic modification may be a good way to overcome acetate overflow, and improve the production of a wide range of acetyl-CoA-derived chemicals.

Improving the production of acetyl-CoA-derived chemicals in Escherichia coli BL21(DE3) through iclR and arcA deletion