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Background  Cyclodipeptides and their derivatives constitute a large class of peptide natural products with noteworthy biological activities. In some yeasts and bacterial species, pulcherriminic acid derived from cyclo-L-leucyl-L-leucyl is excreted and chelates free ferric ions to form the pulcherrimin. In  Bacillus subtilis , the enzymes YvmC and CypX are known to be involved in pulcherriminic acid biosynthesis. However, the mechanisms controlling the transcription of the  yvmC - cypX  operon are still unknown.    Results  In this work, we demonstrated that the  B. subtilis  YvmB MarR-like regulator is the major transcription factor controlling  yvmC - cypX  expression. A comprehensive quantitative proteomic analysis revealed a wide and prominent effect of  yvmB  deletion on proteins involved in cellular processes depending on iron availability. In addition, expression of  yvmB  depends on iron availability. Further analysis with real-time  in vivo  transcriptional profiling allowed us to define the YvmB regulon. We identified  yvmBA ,  yvmC-cypX  and  yvnB  for negative regulation and  yisI  for positive regulation. In combination with genetic approaches, gel mobility shift assays indicated that a 14-bp palindromic motif constitutes the YvmB binding site. It was unexpected that YvmB controls expression of  yisI , whose encoding protein plays a negative role in the regulation of the sporulation initiation pathway. YvmB appears as an additional regulatory element into the cell’s decision to grow or sporulate.    Conclusion  Our findings reveal a possible role of the  B. subtilis  YvmB regulator in the regulatory networks connected to iron metabolism and to the control of proper timing of sporulation. YvmB was renamed as PchR controlling the pulcherriminic acid biosynthetic pathway of  B. subtilis .    Electronic supplementary material  The online version of this article (doi:10.1186/s12866-016-0807-3) contains supplementary material, which is available to authorized users.

bmc microbiology

The MarR-like protein PchR (YvmB) regulates expression of genes involved in pulcherriminic acid biosynthesis and in the initiation of sporulation in Bacillus subtilis