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Background  The signaling second messenger cyclic AMP (cAMP) regulates many aspects of cellular function in all organisms. Previous studies have suggested a role for cAMP in the regulation of gene expression of cellulolytic enzymes in  Trichoderma reesei  (anamorph of  Hypocrea jecorina ).    Methods  The effects of cAMP in T. reesei were analyzed through both activity and expression of cellulase, intracellular cAMP level measurement, western blotting, indirect immunofluorescence and confocal microscopy.    Results  To elucidate the involvement of cAMP in the cellulase expression, we analyzed the growth of the mutant strain ∆ acy1  and its parental strain QM9414 in the presence of the inducers cellulose, cellobiose, lactose, or sophorose, and the repressor glucose. Our results indicated that cAMP regulates the expression of cellulase in a carbon source-dependent manner. The expression  cel7a,  and  cel6a  genes was higher in the presence of sophorose than in the presence of cellulose, lactose, cellobiose, or glucose. Moreover, intracellular levels of cAMP were up to four times higher in the presence of sophorose compared to other carbon sources. Concomitantly, our immunofluorescence microscopy and western blot data suggest that in the presence of sophorose, cAMP may regulate secretion of cellulolytic enzymes in  T. reesei .    Conclusions  These results allow us to better understand the role of cAMP and expand our knowledge on the signal transduction pathways involved in the regulation of cellulase expression in  T. reesei.  Finally, our data may help develop new strategies to improve the expression of  cel7a  and  cel6a  genes, and therefore, favor their application in several biotechnology fields.    Electronic supplementary material  The online version of this article (doi:10.1186/s12866-015-0536-z) contains supplementary material, which is available to authorized users.

Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose