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Background  The  pstSCAB  operon of  Corynebacterium glutamicum , which encodes an ABC transport system for uptake of phosphate (P i ), is induced during the P i  starvation response. The two-component regulatory system PhoRS is involved in this response, but partial P i  starvation induction of  pstSCAB  in a Δ phoRS  mutant indicated the involvement of additional regulator(s). Regulation of  pstSCAB  also involves the global transcriptional regulator GlxR.    Results  DNA affinity chromatography identified the regulator of acetate metabolism RamB as a protein binding to  pstS  promoter DNA  in vitro . Gel mobility shift assays and mutational analysis of the  pstS  promoter region revealed that RamB binds to two sites localized at positions −74 to −88 and −9 to +2 with respect to the transcriptional start site of  pstSCAB . Reporter gene studies supported the  in vivo  relevance of both binding sites for activation of  pstSCAB  by RamB. DNA microarray analysis revealed that expression of many P i  starvation genes reached higher levels during the P i  starvation response on minimal medium with glucose as sole carbon source than in P i  starved acetate-grown  C. glutamicum  cells.    Conclusions  In  C. glutamicum , RamB is involved in expression control of  pstSCAB  operon. Thus, transcriptional regulation of  pstSCAB  is complex involving activation by the phosphate-responsive two-component regulatory system PhoSR and the regulators of carbon metabolism GlxR and RamB.

Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB