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Background Long noncoding RNAs (lncRNAs) are involved in physiological and pathological processes. However, no studies have been conducted on the relationship between lncRNAs and renal aging. Results First, we evaluated the histopathology of young (3-month-old) and old (24-month-old) C57BL/6J mouse kidneys. Masson trichrome staining and PAS staining showed interstitial collagen deposition and fibrosis, mesangial matrix expansion, a thicker basement membrane and renal interstitial fibrosis in old mouse kidneys. Senescence-associated β-galactosidase (SA-β-gal)-positive areas in the kidneys of old mice were significantly elevated compared to those of young mice. Then, we analyzed the differential expression of lncRNAs and mRNAs in the kidneys of young and old mouse kidneys by RNA-seq analysis. 42 known and 179 novel differentially expressed lncRNAs and 702 differential mRNAs were detected in the mouse kidney. Next, we focused on the differentially expressed mRNAs and lncRNAs by RNA-seq. GO and KEGG analyses were performed based on differentially expressed mRNAs between young and old mouse kidneys. Transregulation based on RIsearch and the correlation coefficient of mRNA-lncRNA were also calculated. The mRNA-lncRNA network was constructed by choosing a Spearman correlation coefficient &gt; 0.9 or &lt;-0.9. GO and KEGG pathway enrichment analyses revealed that differentially expressed mRNAs participated in aging-related pathways. A total of 10 lncRNAs and  trans -regulated mRNAs were constructed. Finally, we validated the role of lncRNA  Gm43360  by CCK-8, flow cytometry, western blot and SA-β-gal staining. The expression level of  Adra1a  was positively correlated and  Csnk1a1  was negatively correlated with lncRNA  Gm43360 . The cell counting kit-8 (CCK-8) results showed that lncRNA  Gm43360  promoted cell viability. LncRNA  Gm43360  increased the percentage of S phase cells and decreased the percentage of G1 phase cells compared with the negative control. LncRNA  Gm43360  decreased the expression of p53, p21 and SA-β-gal. Conclusions LncRNA  Gm43360  may play a protective role in kidney aging.

Predict the role of lncRNA in kidney aging based on RNA sequencing