English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background   Monochamus alternatus  Hope is one of the insect vectors of pinewood nematode ( Bursaphelenchus xylophilus ), which causes the destructive pine wilt disease. The microorganisms within the ecosystem, comprising plants, their environment, and insect vectors, form complex networks. This study presents a systematic analysis of the bacterial microbiota in the  M. alternatus  midgut and its habitat niche.    Methods  Total DNA was extracted from 20 types of samples (with three replicates each) from  M. alternatus  and various tissues of healthy and infected  P. massoniana  (pines). 16S rDNA amplicon sequencing was conducted to determine the composition and diversity of the bacterial microbiota in each sample. Moreover, the relative abundances of bacteria in the midgut of  M. alternatus  larvae were verified by counting the colony-forming units.    Results  Pinewood nematode infection increased the microbial diversity in pines.  Bradyrhizobium ,  Burkholderia ,  Dyella ,  Mycobacterium , and  Mucilaginibacter  were the dominant bacterial genera in the soil and infected pines. These results indicate that the bacterial community in infected pines may be associated with the soil microbiota. Interestingly, the abundance of the genus  Gryllotalpicola  was highest in the bark of infected pines. The genus  Cellulomonas  was not found in the midgut of  M. alternatus , but it peaked in the phloem of infected pines, followed by the phloem of heathy pines. Moreover, the genus  Serratia  was not only present in the habitat niche, but it was also enriched in the  M. alternatus  midgut. The colony-forming unit assays showed that the relative abundance of  Serratia  sp. peaked in the midgut of instar II larvae (81%).    Conclusions  Overall, the results indicate that the bacterial microbiota in the soil and in infected pines are correlated. The  Gryllotalpicola  sp. and  Cellulomonas  sp. are potential microbial markers of pine wilt disease. Additionally,  Serratia  sp. could be an ideal agent for expressing insecticidal protein in the insect midgut by genetic engineering, which represents a new use of microbes to control  M. alternatus .

bmc genomics

Characterization of bacterial communities associated with the pinewood nematode insect vector Monochamus alternatus Hope and the host tree Pinus massoniana