English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Bacterial blotch is a group of economically important diseases affecting the cultivation of common button mushroom,  Agaricus bisporus . Despite being studied for more than a century, the identity and nomenclature of blotch-causing  Pseudomonas  species is still unclear. This study aims to molecularly characterize the phylogenetic and phenotypic diversity of blotch pathogens in Western Europe.    Methods  In this study, blotched mushrooms were sampled from farms across the Netherlands, United Kingdom and Belgium. Bacteria were isolated from symptomatic cap tissue and tested in pathogenicity assays on fresh caps and in pots. Whole genome sequences of pathogenic and non-pathogenic isolates were used to establish phylogeny via multi-locus sequence alignment (MLSA), average nucleotide identity (ANI) and in-silico DNA:DNA hybridization (DDH) analyses.    Results  The known pathogens “ Pseudomonas gingeri ”,  P. tolaasii , “ P. reactans ” and  P. costantinii  were recovered from blotched mushroom caps. Seven novel pathogens were also identified, namely,  P. yamanorum, P. edaphica, P. salomonii  and strains that clustered with  Pseudomonas  sp.  NC02  in one genomic species ,  and three non-pseudomonads, i.e.  Serratia liquefaciens, S. proteamaculans  and a  Pantoea  sp. Insights on the pathogenicity and symptom severity of these blotch pathogens were also generated.    Conclusion  A detailed overview of genetic and regional diversity and the virulence of blotch pathogens in Western Europe, was obtained via the phylogenetic and phenotypic analyses. This information has implications in the study of symptomatic disease expression, development of diagnostic tools and design of localized strategies for disease management.

Molecular characterization of Pseudomonas from Agaricus bisporus caps reveal novel blotch pathogens in Western Europe