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Background  Plant biomass is the most abundant carbon source for many fungal species. In the biobased industry fungi, are used to produce lignocellulolytic enzymes to degrade agricultural waste biomass. Here we evaluated if it would be possible to create an  Aspergillus nidulans  strain that releases, but does not metabolize hexoses from plant biomass. For this purpose, metabolic mutants were generated that were impaired in glycolysis, by using hexokinase ( hxkA ) and glucokinase ( glkA ) negative strains. To prevent repression of enzyme production due to the hexose accumulation, strains were generated that combined these mutations with a deletion in  creA , the repressor involved in regulating preferential use of different carbon catabolic pathways.    Results  Phenotypic analysis revealed reduced growth for the  hxkA1 glkA4  mutant on wheat bran. However, hexoses did not accumulate during growth of the mutants on wheat bran, suggesting that glucose metabolism is re-routed towards alternative carbon catabolic pathways. The  creAΔ4  mutation in combination with preventing initial phosphorylation in glycolysis resulted in better growth than the  hxkA/glkA  mutant and an increased expression of pentose catabolic and pentose phosphate pathway genes. This indicates that the reduced ability to use hexoses as carbon sources created a shift towards the pentose fraction of wheat bran as a major carbon source to support growth.    Conclusion  Blocking the direct entry of hexoses to glycolysis activates alternative metabolic conversion of these sugars in  A. nidulans  during growth on plant biomass, but also upregulates conversion of other sugars, such as pentoses.    Electronic supplementary material  The online version of this article (10.1186/s12864-018-4609-x) contains supplementary material, which is available to authorized users.

bmc genomics

Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans