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Background    Fragaria vesca  or ‘woodland strawberry’ has emerged as an attractive model for the study of ripening of non-climacteric fruit. It has several advantages, such as its small genome and its diploidy. The recent availability of the complete sequence of its genome opens the possibility for further analysis and its use as a reference species. Fruit softening is a physiological event and involves many biochemical changes that take place at the final stages of fruit development; among them, the remodeling of cell walls by the action of a set of enzymes. Xyloglucan endotransglycosylase/hydrolase (XTH) is a cell wall-associated enzyme, which is encoded by a multigene family. Its action modifies the structure of xyloglucans, a diverse group of polysaccharides that crosslink with cellulose microfibrills, affecting therefore the functional structure of the cell wall. The aim of this work is to identify the XTH-encoding genes present in  F. vesca  and to determine its transcription level in ripening fruit.    Results  The search resulted in identification of 26 XTH-encoding genes named as  FvXTH s. Genetic structure and phylogenetic analyses were performed allowing the classification of  FvXTH  genes into three phylogenetic groups: 17 in group I/II, 2 in group IIIA and 4 in group IIIB. Two sequences were included into the ancestral group. Through a comparative analysis, characteristic structural protein domains were found in FvXTH protein sequences. In complement, expression analyses of  FvXTHs  by qPCR were performed in fruit at different developmental and ripening stages, as well as, in other tissues. The results showed a diverse expression pattern of  FvXTH s in several tissues, although most of them are highly expressed in roots. Their expression patterns are not related to their respective phylogenetic groups. In addition, most  FvXTH s are expressed in ripe fruit, and interestingly, some of them ( FvXTH 18  and  20 , belonging to phylogenic group I/II, and  FvXTH 25  and  26  to group IIIB) display an increasing expression pattern as the fruit ripens.    Conclusion  A discrete group of  FvXTHs  ( 18, 20 ,  25  and  26)  increases their expression during softening of  F. vesca  fruit, and could take part in cell wall remodeling required for softening in collaboration with other cell wall degrading enzymes.    Electronic supplementary material  The online version of this article (10.1186/s12864-017-4255-8) contains supplementary material, which is available to authorized users.

XTHs from Fragaria vesca: genomic structure and transcriptomic analysis in ripening fruit and other tissues