English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Background    Phlomis  plants are a source of biological active substances with potential applications in the control of phytopathogens.  Phlomis purpurea  (Lamiaceae) is autochthonous of southern Iberian Peninsula and Morocco and was found to be resistant to  Phytophthora cinnamomi. Phlomis purpurea  has revealed antagonistic effect in the rhizosphere of  Quercus suber  and  Q. ilex  against  P. cinnamomi .  Phlomis purpurea  roots produce bioactive compounds exhibiting antitumor and anti- Phytophthora  activities with potential to protect susceptible plants. Although these important capacities of  P. purpurea  have been demonstrated, there is no transcriptomic or genomic information available in public databases that could bring insights on the genes underlying this anti-oomycete activity.    Results  Using Illumina technology we obtained a de novo assembly of  P. purpurea  transcriptome and differential transcript abundance to identify putative defence related genes in challenged versus non-challenged plants. A total of 1,272,600,000 reads from 18 cDNA libraries were merged and assembled into 215,739 transcript contigs .  BLASTX alignment to Nr NCBI database identified 124,386 unique annotated transcripts (57.7%) with significant hits. Functional annotation identified 83,550 out of 124,386 unique transcripts, which were mapped to 141 pathways.  39% of unigenes were assigned GO terms. Their functions cover biological processes, cellular component and molecular functions. Genes associated with response to stimuli, cellular and primary metabolic processes, catalytic and transporter functions were among those identified.  Differential transcript abundance analysis using DESeq revealed significant differences among libraries depending on post-challenge times.  Comparative cyto-histological studies of  P. purpurea  roots challenged with  P. cinnamomi  zoospores and controls revealed specific morphological features (exodermal strips and epi-cuticular layer), that may provide a constitutive efficient barrier against pathogen penetration. Genes involved in cutin biosynthesis and in exodermal Casparian strips formation were up-regulated.    Conclusions  The de novo assembly of transcriptome using short reads for a non-model plant,  P. purpurea , revealed many unique transcripts useful for further gene expression, biological function, genomics and functional genomics studies.  The data presented suggest a combination of a constitutive resistance and an increased transcriptional response from  P. purpurea  when challenged with the pathogen .  This knowledge opens new perspectives for the understanding of defence responses underlying pathogenic oomycete/plant interaction upon challenge with  P. cinnamomi .    Electronic supplementary material  The online version of this article (doi:10.1186/s12864-017-4042-6) contains supplementary material, which is available to authorized users.

De novo assembly of Phlomis purpurea after challenging with Phytophthora cinnamomi