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Background    Aedes albopictus  is a vector of arboviruses that cause severe diseases in humans such as Chikungunya, Dengue and Zika fevers. The vector competence of  Ae. albopictus  varies depending on the mosquito population involved and the virus transmitted.  Wolbachia  infection status in believed to be among key elements that determine viral transmission efficiency. Little is known about the cellular functions mobilized in  Ae. albopictus  during co-infection by  Wolbachia  and a given arbovirus. To decipher this tripartite interaction at the molecular level, we performed a proteome analysis in  Ae. albopictus  C6/36 cells mono-infected by  Wolbachia w AlbB strain or Chikungunya virus (CHIKV), and bi-infected.    Results  We first confirmed significant inhibition of CHIKV by  Wolbachia . Using two-dimensional gel electrophoresis followed by nano liquid chromatography coupled with tandem mass spectrometry, we identified 600 unique differentially expressed proteins mostly related to glycolysis, translation and protein metabolism.  Wolbachia  infection had greater impact on cellular functions than CHIKV infection, inducing either up or down-regulation of proteins associated with metabolic processes such as glycolysis and ATP metabolism, or structural glycoproteins and capsid proteins in the case of bi-infection with CHIKV. CHIKV infection inhibited expression of proteins linked with the processes of transcription, translation, lipid storage and miRNA pathways.    Conclusions  The results of our proteome profiling have provided new insights into the molecular pathways involved in tripartite  Ae. albopictus - Wolbachia -CHIKV interaction and may help defining targets for the better implementation of  Wolbachia -based strategies for disease transmission control.    Electronic supplementary material  The online version of this article (doi:10.1186/s12864-017-3985-y) contains supplementary material, which is available to authorized users.

Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon