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Background  Acarbose is used in the treatment of diabetes mellitus type II and is produced by  Actinoplanes  sp. SE50/110. Although the biosynthesis of acarbose has been intensively studied, profound knowledge about transcription factors involved in acarbose biosynthesis and their binding sites has been missing until now. In contrast to acarbose biosynthetic gene clusters in  Streptomyces  spp., the corresponding gene cluster of  Actinoplanes  sp. SE50/110 lacks genes for transcriptional regulators.    Results  The acarbose regulator C (AcrC) was identified through an in silico approach by aligning the LacI family regulators of acarbose biosynthetic gene clusters in  Streptomyces  spp. with the  Actinoplanes  sp. SE50/110 genome. The gene for  acrC , located in a head-to-head arrangement with the maltose/maltodextrin ABC transporter  malEFG  operon, was deleted by introducing PCR targeting for  Actinoplanes  sp. SE50/110. Characterization was carried out through cultivation experiments, genome-wide microarray hybridizations, and RT-qPCR as well as electrophoretic mobility shift assays for the elucidation of binding motifs. The results show that AcrC binds to the intergenic region between  acbE  and  acbD  in  Actinoplanes  sp. SE50/110 and acts as a transcriptional repressor on these genes. The transcriptomic profile of the wild type was reconstituted through a complementation of the deleted  acrC  gene. Additionally, regulatory sequence motifs for the binding of AcrC were identified in the intergenic region of  acbE  and  acbD . It was shown that AcrC expression influences acarbose formation in the early growth phase. Interestingly, AcrC does not regulate the  malEFG  operon.    Conclusions  This study characterizes the first known transcription factor of the acarbose biosynthetic gene cluster in  Actinoplanes  sp. SE50/110. It therefore represents an important step for understanding the regulatory network of this organism. Based on this work, rational strain design for improving the biotechnological production of acarbose can now be implemented.    Electronic supplementary material  The online version of this article (doi:10.1186/s12864-017-3941-x) contains supplementary material, which is available to authorized users.

The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110