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Background  Zinc finger proteins (ZFPs) play an important role in regulating plant responses to abiotic stress. However, little is known about the function of LSD1-like-type ZFP in saline-alkaline (SA) stress resistance of rice. In this study,  OsLOL5  (GenBank No. AJ620677), containing two LSD1-like-type C2C2 domains, was isolated and analyzed its protection roles in transgenic plants and yeast.  OsLOL5  was located in the nucleus as evidenced by the bombardment of onion epidermal cells.    Results   OsLOL5  expression significantly increased in rice leaves and roots under 150 mmol L-1 NaCl, 30 mM NaHCO 3 , and 10 mmol L-1 H 2 O 2  treatment, respectively. Overexpression of  OsLOL5  in yeast resulted in SA tolerance at significant level. Transgenic Arabidopsis plants overexpressing  OsLOL5  grew well in the presence ofboth NaCl and NaHCO 3  treatments, whereas wild-type plants exhibited chlorosis, stunted growth phenotype, and even death. SA stress caused significant changes in the malondialdehyde (MDA) contents in non-transgenic plants compared with those in transgenic lines. Transgenic rice overexpressing  OsLOL5  exhibited stronger resistance than NT under NaHCO 3  treatment, as demonstrated by its greater shoot length, and fresh weight. The genes associated with oxidative stress, such as  OsAPX2 ,  OsCAT ,  OsCu/Zn-SOD , and  OsRGRC2 , were significantly upregulated in  OsLOL5 -overexpressing rice. The results suggested that  OsLOL5  improved SA tolerance in plants, and regulated oxidative and salinity stress retardation via the active oxygen detoxification pathway.    Conclusions  The yeast INVScI bacterium grew significantly better than the control strain under NaCl, NaHCO 3 , and H 2 O 2  treatments. These findings illustrated that  OsLOL5  overexpression enhanced yeast resistance for SA stress through active oxygen species. The present study showed that the  OsLOL5  genes involved in the ROS signaling pathways may combine with the model plant Arabidopsis and rice in LDS1-type ZFP by ROS signaling pathways that regulate cell necrosis. We speculated that the  OsLOL5  active oxygen scavenging system may have coordinating roles. The present study further revealed that  OsLOL5  ZFP could regulate oxidative stress function, but could also provide a basis for salt-resistant rice strains.    Electronic supplementary material  The online version of this article (doi:10.1186/s12864-016-2460-5) contains supplementary material, which is available to authorized users.

A rice LSD1-like-type ZFP gene OsLOL5 enhances saline-alkaline tolerance in transgenic Arabidopsis thaliana, yeast and rice