English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The bacterial species  Xanthomonas campestris  infects a wide range of  Brassicaceae . Specific pathovars of this species cause black rot (pv.  campestris ), bacterial blight of stock (pv.  incanae ) or bacterial leaf spot (pv.  raphani ).    Results  In this study, we extended the genomic coverage of the species by sequencing and annotating the genomes of strains from pathovar  incanae  (CFBP 1606R and CFBP 2527R), pathovar  raphani  (CFBP 5828R) and a pathovar formerly named  barbareae  (CFBP 5825R). While comparative analyses identified a large core ORFeome at the species level, the core type III effectome was limited to only three putative type III effectors (XopP, XopF1 and XopAL1). In  Xanthomonas , these effector proteins are injected inside the plant cells by the type III secretion system and contribute collectively to virulence. A deep and strand-specific RNA sequencing strategy was adopted in order to experimentally refine genome annotation for strain CFBP 5828R. This approach also allowed the experimental definition of novel ORFs and non-coding RNA transcripts. Using a constitutively active allele of  hrpG , a master regulator of the type III secretion system, a HrpG-dependent regulon of 141 genes co-regulated with the type III secretion system was identified. Importantly, all these genes but seven are positively regulated by HrpG and 56 of those encode components of the Hrp type III secretion system and putative effector proteins.    Conclusions  This dataset is an important resource to mine for novel type III effector proteins as well as for bacterial genes which could contribute to pathogenicity of  X. campestris .    Electronic supplementary material  The online version of this article (doi:10.1186/s12864-015-2190-0) contains supplementary material, which is available to authorized users.

Genomics and transcriptomics of Xanthomonas campestris species challenge the concept of core type III effectome