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Background  DapuNPV ( Dasychira pudibunda  nucleopolyhedrovirus), presented in this report, belongs to  Alphabaculovirus  group Ib. Its full, newly sequenced genome shows close relationship to baculovirus OpMNPV isolated from douglas-fir tussock moth  Orgyia pseudotsugata . Baculovirus DapuNPV is a natural limiter of pale tussock moth  Dasychira pudibunda  L. (syn.  Calliteara pudibunda  L.)(Lepidoptera,  Lymantriidae ), which can occur in a form of an outbreak on many species of deciduous trees and may cause significant economic losses in the forests.    Methods  Late instars dead larvae of pale tussock moth were mechanically homogenized and polyhedra were purified during series of ultracentrifugation. Viral DNA was extarcted and sequenced using Miseq Illumina platform. 294,902 paired reads were used for de novo assembling. Genome annotation, multiple allingment to others baculoviruses and phylogegentic analises were perform with the use of multiple bioinformatic tools like: Glimmer3, HMMER web server, Geneious 7 and MEGA6.    Results  The genome of DapuNPV is 136,761 bp long with AT pairs content 45.6 %. The predicted number of encoded putative open reading frames (ORFs) is 161 and six of them demonstrate low or no homology to ORFs previously found in baculoviruses. DapuNPV genome shows very high similarity to OpMNPV in a nucleotide sequence (91.1 % of identity) and gene content (150 homologous ORFs), though some major differences (e.g. lack of he65 in OpMNPV) have also been noted.    Conclusions  Similarly to other members of the  Baculoviridae  family, DapuNPV baculovirus possesses highly conserved core genes. Among them, there is a second copy of occluded derived virus envelope 27 protein ( odv-e27 ), which was previously found only in a member of  Alphabaculovirus  group II – LyxyMNPV ( Lymantria xylina  MNPV). Surprisingly enough, DapuNPV and LyxyMNPV genomes share also another feature. Phylogenetic analysis of chitin binding family protein ( cbpl ) indicates significant similarity of those two baculoviruses from distinct evolutionary groups which infect the same hosts from  Lymantriidae . The ubiquitin like family gene ( ubil ), which has not been described until now, is another characteristic component of DapuNPV genome.    Electronic supplementary material  The online version of this article (doi:10.1186/s12864-015-1963-9) contains supplementary material, which is available to authorized users.

bmc genomics

The genome of Dasychira pudibunda nucleopolyhedrovirus (DapuNPV) reveals novel genetic connection between baculoviruses infecting moths of the Lymantriidae family