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Background  Piscirickettsiosis or Salmonid Rickettsial Septicaemia (SRS) is a bacterial disease that has a major economic impact on the Chilean salmon farming industry. Despite the fact that Piscirickettsia salmonis has been recognized as a major fish pathogen for over 20 years, the molecular strategies underlying the fish response to infection and the bacterial mechanisms of pathogenesis are poorly understood. We analysed and compared the head kidney transcriptional response of Atlantic salmon (Salmo salar) families with different levels of susceptibility to P. salmonis infection in order to reveal mechanisms that might confer infection resistance.    Results  We ranked forty full-sibling Atlantic salmon families according to accumulated mortality after a challenge with  P. salmonis  and selected the families with the lowest and highest cumulative mortalities for microarray gene expression analysis. A comparison of the response to  P. salmonis  infection between low and high susceptibility groups identified biological processes presumably involved in natural resistance to the pathogen. In particular, expression changes of genes linked to cellular iron depletion, as well as low iron content and bacterial load in the head kidney of fish from low susceptibility families, suggest that iron-deprivation is an innate immunity defence mechanism against  P. salmonis . To complement these results, we predicted a set of iron acquisition genes from the  P. salmonis  genome. Identification of putative Fur boxes and expression of the genes under iron-depleted conditions revealed that most of these genes form part of the Fur regulon of  P. salmonis.Conclusions  This study revealed, for the first time, differences in the transcriptional response to  P. salmonis  infection among Atlantic salmon families with varied levels of susceptibility to the infection. These differences correlated with changes in the abundance of transcripts encoding proteins directly and indirectly involved in the immune response; changes that highlighted the role of nutritional immunity through iron deprivation in host defence mechanisms against  P. salmonis . Additionally, we found that  P. salmonis  has several mechanisms for iron acquisition, suggesting that this bacterium can obtain iron from different sources, including ferric iron through capturing endogenous and exogenous siderophores and ferrous iron. Our results contribute to determining the underlying resistance mechanisms of Atlantic salmon to  P. salmonis  infection and to identifying future treatment strategies.    Electronic supplementary material  The online version of this article (doi:10.1186/s12864-015-1716-9) contains supplementary material, which is available to authorized users.

bmc genomics

Transcriptional response of Atlantic salmon families to Piscirickettsia salmonis infection highlights the relevance of the iron-deprivation defence system