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Background   Bactrocera dorsalis  is a destructive polyphagous and highly invasive insect pest of tropical and subtropical species of fruit and vegetable crops. The sterile insect technique (SIT) has been used for decades to control insect pests of agricultural, veterinary, and human health importance. Irradiation of pupae in SIT can reduce the ecological fitness of the sterile insects. Our previous study has shown that a gut bacterial strain BD177 that could restore ecological fitness by promoting host food intake and metabolic activities.    Results  Using long-read sequence technologies, we assembled the complete genome of  K. michiganensis  BD177 strain. The complete genome of  K. michiganensis  BD177 comprises one circular chromosome and four plasmids with a GC content of 55.03%. The pan-genome analysis was performed on 119 genomes (strain BD177 genome and 118 out of 128 published  Klebsiella sp.  genomes since ten were discarded). The pan-genome includes a total of 49305 gene clusters, a small number of 858 core genes, and a high number of accessory (10566) genes. Pan-genome and average nucleotide identity (ANI) analysis showed that BD177 is more similar to the type strain  K. michiganensis  DSM2544, while away from the type strain  K. oxytoca  ATCC13182. Comparative genome analysis with 21  K. oxytoca  and 12  K. michiganensis  strains, identified 213 unique genes, several of them related to amino acid metabolism, metabolism of cofactors and vitamins, and xenobiotics biodegradation and metabolism in BD177 genome.    Conclusions  Phylogenomics analysis reclassified strain BD177 as a member of the species  K. michiganensis.  Comparative genome analysis suggested that  K. michiganensis  BD177 has the strain-specific ability to provide three essential amino acids (phenylalanine, tryptophan and methionine) and two vitamins B (folate and riboflavin) to  B. dorsalis.  The clear classification status of BD177 strain and identification of unique genetic characteristics may contribute to expanding our understanding of the symbiotic relationship of gut microbiota and  B. dorsalis .    Supplementary Information  The online version contains supplementary material available at 10.1186/s12863-020-00945-0.

bmc genomic data

Comparative genomics of Klebsiella michiganensis BD177 and related members of Klebsiella sp. reveal the symbiotic relationship with Bactrocera dorsalis