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Background  Glycosphingolipids (GSLs) are important membrane components composed of a carbohydrate structure attached to a hydrophobic ceramide. They can serve as specific membrane receptors for microbes and microbial products, such as F4  Escherichia coli  (F4 ETEC) and isolated F4 fimbriae. The aim of this study was to investigate the hypothesis that variation in genes involved in the assembly of the F4 binding carbohydrate moiety of GSLs (i.e.  ARSA, B4GALT6, GAL3ST1, GALC, GBA, GLA, GLB1, GLB1L, NEU1, NEU2, UGCG, UGT8 ) could account for differential binding of F4 ETEC and their fimbriae.    Results  RT-PCR could not reveal any differential expression of the 12 genes in the jejunum of F4 receptor-positive (F4R + ) and F4 receptor-negative (F4R - ) pigs. Sequencing the complete open reading frame of the 11 expressed genes ( NEU2  was not expressed) identified 72 mutations. Although some of them might have a structural effect, none of them could be associated with a F4R phenotype.    Conclusion  We conclude that no regulatory or structural variation in any of the investigated genes is responsible for the genetic susceptibility of pigs towards F4 ETEC.    Electronic supplementary material  The online version of this article (doi:10.1186/s12863-014-0103-x) contains supplementary material, which is available to authorized users.

bmc genomic data

Variation in 12 porcine genes involved in the carbohydrate moiety assembly of glycosphingolipids does not account for differential binding of F4 Escherichia coliand their fimbriae