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Background  Prostate androgen-regulated mucin-like protein 1 (PARM1) is a pro-proliferative and anti-apoptotic glycoprotein involved in the endoplasmic reticulum (ER) stress response. A single nucleotide polymorphism in the coding region of  PARM1  has been associated with competence of bovine embryos to develop to the blastocyst stage. Here we tested the importance of PARM1 for development by evaluating consequences of reducing  PARM1  mRNA abundance on embryonic development and differentiation, gene expression and resistance to ER stress.    Results  Knockdown of  PARM1  using an anti- PARM1  GapmeR did not affect competence of embryos to develop into blastocysts but decreased the number of trophectoderm (TE) cells in the blastocyst and tended to increase the number of cells in the blastocyst inner cell mass (ICM). Treatment of embryos with anti- PARM1  GapmeR affected expression of 4 and 3 of 90 genes evaluated at the compact-morula and blastocyst stage of development at days 5.5 and 7.5 after fertilization, respectively. In morulae, treatment increased expression of  DAB2, INADL,  and  STAT3  and decreased expression of  CCR2 . At the blastocyst stage, knockdown of  PARM1  increased expression of  PECAM  and  TEAD4  and decreased expression of  CCR7.  The potential role of PARM1 in ER stress response was determined by evaluating effects of knockdown of  PARM1  on development of embryos after exposure to heat shock or tunicamycin and on expression of  ATF6 ,  DDIT3  and  EIF2AK3  at the compact morula and blastocyst stages. Both heat shock and tunicamycin reduced the percent of embryos becoming a blastocyst but response was unaffected by  PARM1  knockdown. Similarly, there was no effect of knockdown on steady-state amounts of  ATF6 ,  DDIT3  or  EIF2AK3.Conclusion   PARM1  participates in formation of TE and ICM cells in early embryonic development but there is no evidence for the role of PARM1 in the ER stress response.    Electronic supplementary material  The online version of this article (10.1186/s12861-019-0195-7) contains supplementary material, which is available to authorized users.

Importance of prostate androgen-regulated mucin-like protein 1 in development of the bovine blastocyst