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Background  It has been suggested that the ectopic expression of PDX1, a dominant pancreatic transcription factor, plays a critical role in the developmental programming of the pancreas even from cells of unrelated tissues such as keratinocytes and amniotic fluid stem cells. In this study we have chosen to drive pancreatic development in human amnion epithelial cells by inducing endogenous  PDX1  expression. Further, we have investigated the role of Epidermal Growth Factor (EGF) and Poly-L-Ornithine (PLO) on this differentiation process.    Results  Human amnion epithelial cells expressed high levels of endogenous  PDX1  upon transduction with an adenoviral vector expressing murine  Pdx1 . Other markers of various stages of pancreatic differentiation such as  NKX6.1 ,  SOX17 ,  RFX6 ,  FOXA2, CFTR ,  NEUROD1 ,  PAX4  and  PPY  were also expressed upon  Pdx1  transduction.  Although initial expression of pancreatic progenitor markers was higher in culture conditions lacking EGF, for a sustained and increased expression EGF was required. Culture on PLO further increased the positive impact of EGF.    Conclusion  Pancreatic marker expression subsequent to m Pdx1  transduction suggests that this approach may facilitate the in vitro differentiation of hAECs into cells of the endocrine pancreas. This result may have important implications in diabetes therapy.    Electronic supplementary material  The online version of this article (doi:10.1186/s12861-016-0108-y) contains supplementary material, which is available to authorized users.

The combined effect of PDX1, epidermal growth factor and poly-L-ornithine on human amnion epithelial cells’ differentiation