English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Chronic leg ulcerations are associated with Haemoglobin disorders, Type2 Diabetes Mellitus, and long-term venous insufficiency, where poor perfusion and altered metabolism develop into a chronic inflammation that impairs wound closure. Skin equivalent organotypic cultures can be engineered in vitro to study skin biology and wound closure by modelling the specific cellular components of the skin. This study aimed to develop a novel bioactive platelet-rich plasma (PRP) leukocyte depleted scaffold to facilitate the study of common clinical skin wounds in patients with poor chronic skin perfusion and low leukocyte infiltration. A scratch assay was performed on the skin model to mimic two skin wound conditions, an untreated condition and a condition treated with recombinant tumour necrotic factor (rTNF) to imitate the stimulation of an inflammatory state. Gene expression of  IL8  and  TGFA  was analysed in both conditions. Statistical analysis was done through ANOVA and paired student t-test.  P  < 0.05 was considered significant.    Results  A skin model that consisted of a leukocyte-depleted, platelet-rich plasma scaffold was setup with embedded fibroblasts as dermal equivalents and seeded keratinocytes as multi-layered epidermis. Gene expression levels of  IL8  and  TGFA  were significantly different between the control and scratched conditions ( p  < 0.001 and  p  < 0.01 respectively), as well as between the control and treated conditions (p < 0.01 and  p  < 0.001 respectively). The scratch assay induced  IL8  upregulation after 3 h ( p  < 0.05) which continued to increase up to day 1 ( p  < 0.05). On the other hand, the administration of TNF led to the downregulation of  IL8  ( p  < 0.01), followed by an upregulation on day 2.  IL8  gene expression decreased in the scratched condition after day 1 as the natural healing process took place and was lower than in the treated condition on day 8 ( p  < 0.05).  Both untreated and treated conditions showed a downregulation of  TGFA  3 h after scratch when compared with the control condition ( p  < 0.01). Administration of rTNF showed significant downregulation of  TGFA  after 24 h when compared with the control ( p  < 0.01) and treated conditions ( p  < 0.05).    Conclusion  This study suggests that a leukocyte-depleted PRP-based skin equivalent can be a useful model for the in vitro study of chronic skin wounds related to poor skin perfusion.    Supplementary Information  The online version contains supplementary material available at 10.1186/s12860-021-00366-6.

Novel leukocyte-depleted platelet-rich plasma-based skin equivalent as an in vitro model of chronic wounds: a preliminary study