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Background  Genotyping-by-sequencing (GBS) has been used broadly in genetic studies for several species, especially those with agricultural importance. However, its use is still limited in autopolyploid species because genotype calling software generally fails to properly distinguish heterozygous classes based on allele dosage.    Results   VCF2SM  is a Python script that integrates sequencing depth information of polymorphisms in variant call format (VCF) files and  SuperMASSA  software for quantitative genotype calling. VCFs can be obtained from any variant discovery software that outputs exact allele sequencing depth, such as a modified version of the  Tassel-GBS  pipeline provided here.  VCF2SM  was successfully applied in analyzing GBS data from diverse panels (alfalfa and potato) and full-sib mapping populations (alfalfa and switchgrass) of polyploid species.    Conclusions  We demonstrate that our approach can help plant geneticists working with autopolyploid species to advance their studies by distinguishing allele dosage from GBS data.    Electronic supplementary material  The online version of this article (10.1186/s12859-018-2433-6) contains supplementary material, which is available to authorized users.

A fully automated pipeline for quantitative genotype calling from next generation sequencing data in autopolyploids