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Background  Identification of individual components in complex mixtures is an important and sometimes daunting task in several research areas like metabolomics and natural product studies. NMR spectroscopy is an excellent technique for analysis of mixtures of organic compounds and gives a detailed chemical fingerprint of most individual components above the detection limit. For the identification of individual metabolites in metabolomics, correlation or covariance between peaks in  1 H NMR spectra has previously been successfully employed. Similar correlation of 2D  1 H- 13 C Heteronuclear Single Quantum Correlation spectra was recently applied to investigate the structure of heparine. In this paper, we demonstrate how a similar approach can be used to identify metabolites in human biofluids (post-prostatic palpation urine).    Results  From 50  1 H- 13 C Heteronuclear Single Quantum Correlation spectra, 23 correlation plots resembling pure metabolites were constructed. The identities of these metabolites were confirmed by comparing the correlation plots with reported NMR data, mostly from the Human Metabolome Database.    Conclusions  Correlation plots prepared by statistically correlating  1 H- 13 C Heteronuclear Single Quantum Correlation spectra from human biofluids provide unambiguous identification of metabolites. The correlation plots highlight cross-peaks belonging to each individual compound, not limited by long-range magnetization transfer as conventional NMR experiments.    Electronic supplementary material  The online version of this article (doi:10.1186/s12859-014-0413-z) contains supplementary material, which is available to authorized users.

Identification of metabolites from 2D 1H-13C HSQC NMR using peak correlation plots