English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Chloroplasts are essential for photosynthesis and play key roles in plant development. High temperature affects structure of chloroplasts and metabolism in plants. The seryl-tRNA synthetase plays an important role in translation of proteins. Although seryl-tRNA synthetase has been widely studied in microbes and animals, few studies have reported about its role in chloroplast development under high temperature in rice.    Results  In this study, we isolated a novel  temperature - sensitive chlorophyll - deficient 11  ( tscd11 ) mutant by ethyl methane sulfonate (EMS) mutagenesis of  japonica  variety Wuyujing7. The  tscd11  mutant developed albino leaves at the 3-leaf stage under high temperature (35 °C), but had normal green leaves under low temperature (25 °C). Consistent with the albino phenotype, impaired chloroplasts, decreased chlorophyll content and increased ROS accumulation were found in the  tscd11  mutant at 35 °C. Fine mapping and DNA sequencing of  tscd11  revealed a missense mutation (G to A) in the eighth exon of  LOC_Os11g39670  resulted in amino acid change (Glu 374  to Lys 374 ). The  TSCD11  gene encodes a seryl-tRNA synthetase localized to chloroplast. Complementation test confirmed that the point mutation in  TSCD11  is responsible for the phenotype of  tscd11 .  TSCD11  is highly expressed in leaves. Compared with the wild type (WT), mutation in  TSCD11  led to significant alteration in expression levels of genes associated with chlorophyll biosynthesis, photosynthesis and chloroplast development under high temperature.    Conclusions   TSCD11 , encoding a seryl-tRNA synthetase localized to chloroplast, is vital to early chloroplast development at high temperature in rice, which help to further study on the molecular mechanism of chloroplast development under high temperature.

Isolation of TSCD11 Gene for Early Chloroplast Development under High Temperature in Rice