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Background  Digalactosyldiacylglycerol (DGDG) is one of the major lipids found predominantly in the photosynthetic membrane of cyanobacteria, eukaryotic algae and higher plants. DGDG, along with MGDG (Monogalactosyldiacylglycerol), forms the matrix in thylakoid membrane of chloroplast, providing the site for photochemical and electron transport reactions of oxygenic photosynthesis.    Results  In silico analysis reveals that rice ( Oryza sativa  L.) genome has 5 genes encoding DGDG synthase, which are differentially expressed in different tissues, and  OsDGD2β  was identified to be the sole DGDG synthase gene expressed in anther. We then developed  osdgd2β  mutants by using the CRISPR/Cas9 system and elucidate its role, especially in the development of anther and pollen. The loss of function of OsDGD2β resulted in male sterility in rice characterized by pale yellow and shrunken anther, devoid of starch granules in pollen, and delayed degeneration of tapetal cells. The total fatty acid and DGDG content in the anther was reduced by 18.66% and 22.72% in  osdgd2β , affirming the importance of DGDG in the development of anther. The mutants had no notable differences in the vegetative phenotype, as corroborated by relative gene expression of DGDG synthase genes in leaves, chlorophyll measurements, and analysis of photosynthetic parameters, implying the specificity of OsDGD2β in anther.    Conclusion  Overall, we showed the importance of DGDG in pollen development and loss of function of OsDGD2β results in male sterility. Here, we have also proposed the use of  OsDGD2β  in hybrid rice breeding using the nuclear male sterility system.    Electronic supplementary material  The online version of this article (10.1186/s12284-019-0320-z) contains supplementary material, which is available to authorized users.

OsDGD2β is the Sole Digalactosyldiacylglycerol Synthase Gene Highly Expressed in Anther, and its Mutation Confers Male Sterility in Rice