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Background  Rice ( Oryza sativa ) is one of the most important food crops in the world. Rice blast, caused by the fungal pathogen  Magnaporthe oryzae , is one of the most destructive rice diseases worldwide. To effectively cope with this problem, the use of rice blast resistance varieties through innovative breeding programs is the best strategy to date. The Thai rice variety Jao Hom Nin (JHN) showed broad-spectrum resistance against Thai rice blast isolates. Two QTLs for blast resistance in JHN were reported on chromosome 1 ( QTL1 ) and 11 ( QTL11 ).    Results  Monogenic lines of  QTL1  (QTL1-C) and  QTL11  (QTL11-C) in the CO39 genetic background were generated. Cluster analysis based on the disease reaction pattern of QTL1-C and QTL11-C, together with IRBLs, showed that those two monogenic lines were clustered with IRBLsh-S ( Pish ) and IRBL7-M ( Pi7 ), respectively. Moreover, sequence analysis revealed that  Pish  and  Pi7  were embedded within the  QTL1  and  QTL11  delimited genomic intervals, respectively. This study thus concluded that  QTL1  and  QTL11  could encode alleles of  Pish  and  Pi7 , designated as  Pish-J  and  Pi7-J , respectively. To validate this hypothesis, the genomic regions of  Pish-J  and  Pi7-J  were cloned and sequenced. Protein sequence comparison revealed that Pish-J and Pi7-J were identical to Pish and Pi7, respectively. The holistic disease spectrum of JHN was found to be exactly attributed to the additive ones of both QTL1-C and QTL11-C.    Conclusion  JHN showed broad spectrum resistance against Thai and Philippine rice blast isolates. As this study demonstrated, the combination of two resistance genes,  Pish-J  and  Pi7-J , in JHN, with each controlling broad-spectrum resistance to rice blast disease, explains the high level of resistance. Thus, the combination of  Pish  and  Pi7  can provide a practical scheme for breeding durable resistance in rice against rice blast disease.    Electronic supplementary material  The online version of this article (doi:10.1186/s12284-017-0159-0) contains supplementary material, which is available to authorized users.

Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast