English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Outer membrane vesicles (OMVs) of  Acinetobacter baumannii  are cytotoxic and elicit a potent innate immune response. OMVs were first identified in  A. baumannii  DU202, an extensively drug-resistant clinical strain. Herein, we investigated protein components of  A. baumannii  DU202 OMVs following antibiotic treatment by proteogenomic analysis.    Methods  Purified OMVs from  A. baumannii  DU202 grown in different antibiotic culture conditions were screened for pathogenic and immunogenic effects, and subjected to quantitative proteomic analysis by one-dimensional electrophoresis and liquid chromatography combined with tandem mass spectrometry (1DE-LC-MS/MS). Protein components modulated by imipenem were identified and discussed.    Results  OMV secretion was increased > twofold following imipenem treatment, and cytotoxicity toward A549 human lung carcinoma cells was elevated. A total of 277 proteins were identified as components of OMVs by imipenem treatment, among which β-lactamase OXA-23, various proteases, outer membrane proteins, β-barrel assembly machine proteins, peptidyl-prolyl cis–trans isomerases and inherent prophage head subunit proteins were significantly upregulated.    Conclusion  In vitro stress such as antibiotic treatment can modulate proteome components in  A. baumannii  OMVs and thereby influence pathogenicity.    Electronic supplementary material  The online version of this article (10.1186/s12014-018-9204-2) contains supplementary material, which is available to authorized users.

clinical proteomics

Antibiotic treatment modulates protein components of cytotoxic outer membrane vesicles of multidrug-resistant clinical strain, Acinetobacter baumannii DU202