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Brucella spp.  are facultative intracellular bacteria that cause zoonotic disease of brucellosis worldwide. Traditional methods for detection of  Brucella spp . take 48–72 h that does not meet the need of rapid detection. Herein, a new rapid detection method of  Brucella  was developed based on polyclonal antibody-conjugating quantum dots and antibody-modified magnetic beads. First, polyclonal antibodies IgG and IgY were prepared and then the antibody conjugated with quantum dots (QDs) and immunomagnetic beads (IMB), respectively, which were activated by  N -(3-dimethylaminopropyl)- N ’-ethylcar-bodiimide hydrochloride (EDC) and  N -hydroxysuccinimide (NHS) to form probes. We used the IMB probe to separate the  Brucella  and labeled by the QD probe, and then detected the fluorescence intensity with a fluorescence spectrometer. The detection method takes 105 min with a limit of detection of 10 3  CFU/mL and ranges from 10 to 10 5  CFU/mL ( R   2  = 0.9983), and it can be well used in real samples.

A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies