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Background  The transcription factor Oct4 plays a pivotal role in the pre-implantation development of the mouse embryo. DNA methyltransferase 1 (Dnmt1) maintains the changes in DNA methylation during mammalian early embryonic development. Little is known of the role of Oct4 in DNA methylation in mice. In this study, Kunming white mice were used as an animal model to reveal any correlation between DNA methylation and Oct4 during mammalian embryonic development.    Results  The expressions of  Dnmt1  and  Oct4  were initially studied using real-time PCR. They exhibited different patterns during the pre-implantation stage. Moreover, by using a promoter assay and ChIP analysis, we found that the transcriptional activities of  Dnmt1  in mouse NIH/3 T3 cells and CCE cells were regulated by Oct4 through direct binding to the − 554 to − 294 fragment of the upstream regulation element of  Dnmt1 . The downregulation of Dnmt1 expression and enzyme activity by mouse Oct4 were further confirmed by transfecting  Oct4  siRNA into mouse CCE cells.    Conclusion  Our results indicate that Oct4 is involved in DNA methylation through the regulation of  Dnmt1  transcription, especially during the early stages of mouse pre-implantation embryo development.    Electronic supplementary material  The online version of this article (10.1186/s11658-018-0104-2) contains supplementary material, which is available to authorized users.

Oct4 regulates DNA methyltransferase 1 transcription by direct binding of the regulatory element