English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The transcription factor TWIST1 plays an important role in the epithelial–mesenchymal transition (EMT) process and in the migration, invasion and metastasis of cancer cells. OCT4, which is a homeobox transcription factor, has an important role in the self-renewal potential of cancer cells. Our aim here is to elucidate impact of ectopic expression of TWIST1 on OCT4 gene expression in esophageal squamous cell carcinoma (ESCC).    Methods  The ESCC line was KYSE30. GP293T cells were transfected with purf-IRES-GFP and pGP plasmids to produce recombinant viral particles. A semi-confluent KYSE30 culture was transduced with the prepared retroviral particles. mRNA extraction and cDNA synthesis were performed from normal KYSE30 cells and those ectopically expressing  TWIST1 . Expressional analysis of  TWIST1  and  OCT4  were performed with relative comparative real-time PCR.    Results  Ectopic expression of  TWIST1  in KYSE30 cells was related to its significant overexpression: nearly nine-fold higher in GFP-h TWIST1  KYSE-30 cells than in control GFP cells. This induced expression of  TWIST1  caused significant upregulation of  OCT4  in GFP-h TWIST1  KYSE-30 cells: nearly eight-fold higher. In silico analysis predicted the correlation of  TWIST1  and  OCT4  through  ETS2 .    Conclusions  Overexpressed  TWIST1  can be correlated with upregulation of the cancer stem cell marker  OCT4  and the protein may play critical regulatory role in  OCT4  gene expression. Since OCT4 is involved in the self-renewal process, the results may suggest a new linkage between  TWIST1  and  OCT4  in the cell biology of ESCC, highlighting the probable role of TWIST1 in inducing self-renewal.

Ectopic expression of TWIST1 upregulates the stemness marker OCT4 in the esophageal squamous cell carcinoma cell line KYSE30