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Metabolites in body fluids reflect multiple biochemical processes and pathways relevant to health and disease. Comprehensive approaches to gain insights into metabolic variation and diseases, such as metabolic phenotyping, have become increasingly popular over recent years [1-3]. These developments have been driven by mass spectrometry (MS) and proton nuclear magnetic resonance (NMR) spectroscopy as the two key experimental technologies. On the basis of findings from multiple disciplines, it has been envisaged that metabolic phenotyping will eventually lead to holistic risk assessment for various diseases [4].

Quantitative high-throughput metabolomics: a new era in epidemiology and genetics