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Map based cloning in  Arabidopsis thaliana  can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel  Arabidopsis  clock mutant  early bird (ebi-1 ) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene  AtNFXL-2  as the likely cause of the  ebi-1  phenotype.

Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis