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The microbiological agar diffusion methods for the detection of antibiotic residues in milk and meat have been used for many years. Various sensitive test-organisms e.g. Micrococcus luteus, Bacillus subtilis, Bacillus cereus, Bacillus stearothermophilus var. calidolactis, etc. have been used in these methods. Some of the methods have been developed as rapid tests, where results can be determined in 2-4 h (Kundrat 1972) , but these methods have so far not been applicable to slaughter-animals. So there is a need for a rapid screening method for the detection of antibiotics in kidneys from slaughter-animals to reveal positive findings before the carcases are ready for dispatch from the slaughterhouses. The screening method described here utilizes the high sensitivity and rapid growth of a Bacillus subtilis strain (a laboratory strain, IMM, B.s. 1) which furthermore is used in a viable count of about 10' per ml of assay agar, or about 100-1000 times more than ordinary test-organisms (Justesen 1973). In order to improve the sulphonamide sensitivity, trimethoprim is added to the medium (Gudding 1976).

A Rapid Agar-Diffusion Test for the Detection of Antibiotic Residues in Kidneys From Slaughter-Animals