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A new alkaline phosphotungstate reduction reaction is introduced in a simple procedure for the determination of the total urate-equivalent chromogens (TUEC) for the first time in serum, aiming that these substances will receive a renewed and well deserved attention. The linear regression equation : Y = 0.0185 + 0.21159 X, the correlation coefficient, r = 0.9975, Sy.x = 0.0075. The Cvs ranged from 0.23 to 2.4% over a concentration range from 2.513 to 4.995 mmol/L. The mean value of TUEC in 18 males was calculated as 3.235 mmol/L, compared to a value of 2.545 mmol/L obtained as the mean TUEC in 18 non-pregnant women.

A new concept for urate in human serum: Colorimetric assay of total urate-equivalent chromogens in human serum