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Methods Rats received cecal-ligation and puncture (CLP) or sham operation, and 24 h later underwent mechanical ventilation for 2.5 h with either VT=9 ml/kg, positive end-expiratory pressure (PEEP)=0 cm H2O (n=9 and n=12, without and with CLP, respectively); or VT=25 ml/kg, PEEP=5 cm H2O (n=13 and n=12, without and with CLP, respectively). Lung tissue samples were obtained and analyzed by nontargeted global fingerprinting approach for lung tissue analysis, applying multiple complementary analytical techniques, including liquid cromatography-mass spectrometry (MS), gas cromatography-MS, and capillary electrophoresis-MS. We followed the Principles of Laboratory Animal Care (2010/63/UE 22-09, RD 53/2013 BOE 1-02, ley 32/2007 BOE 7-11).

0854. Metabolomic changes by mass spectrometry in lung tissue from septic rats with mechanical ventilation-induced lung injury