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Acremonium cellulolyticus  is one of several fungi that offer promise as an alternative to  Trichoderma reesei  for use in industrial cellulase production. However, the mechanism of cellulase production has not been studied at the molecular level because adequate genetic engineering tools for use in  A. cellulolyticus  are lacking. In the present study, we developed a gene disruption method for  A. cellulolyticus , which needs a longer homologous region length. We cloned a putative  A. cellulolyticus creA  gene that is highly similar to the  creA  genes derived from other filamentous fungi, and isolated a  creA  disruptant strain by using the disruption method. Growth of the  creA  disruptant on agar plates was slower than that of the control strain. In the wild-type strain, the CreA protein was localized in the nucleus, suggesting that the cloned gene encodes the CreA transcription factor. Cellulase and xylanase production by the  creA  disruptant were higher than that of the control strain at the enzyme and transcription levels. Furthermore, the  creA  disruptant produced cellulase and xylanase in the presence of glucose. These data suggest both that the CreA protein functions as a catabolite repressor protein, and that disruption of  creA  is effective for enhancing enzyme production by  A. cellulolyticus .

Enhancing cellulase and hemicellulase production by genetic modification of the carbon catabolite repressor gene, creA, in Acremonium cellulolyticus