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2,5-diketo-D-gluconic acid reductase (2,5-DKG reductase) catalyses the reduction of 2,5-diketo-D-gluconic acid (2,5-DKG) to 2-keto-L-gulonic acid (2-KLG), a direct precursor (lactone) of L-ascorbic acid (vitamin C). This reaction is an essential step in the biocatalytic production of the food supplement vitamin C from D-glucose or D-gluconic acid. As 2,5-DKG reductase is usually produced recombinantly, it is of interest to establish an efficient process for 2,5-DKG reductase production that also satisfies food safety requirements. In the present study, three recently described food grade variants of the  Lactobacillales  based expression systems pSIP ( Lactobacillus plantarum ) and NICE ( Lactococcus lactis ) were evaluated with regard to their effictiveness to produce 2,5-DKG reductase from  Corynebacterium glutamicum . Our results indicate that both systems are suitable for 2,5-DKG reductase expression. Maximum production yields were obtained with  Lb. plantarum /pSIP609 by pH control at 6.5. With 262 U per litre of broth, this represents the highest heterologous expression level so far reported for 2,5-DKG reductase from  C. glutamicum . Accordingly,  Lb. plantarum/ pSIP609 might be an interesting alternative to  Escherichia coli  expression systems for industrial 2,5-DKG reductase production.

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Evaluation of the food grade expression systems NICE and pSIP for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum