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Lactobacillus plantarum  is involved in a multitude of food related industrial fermentation processes including the malolactic fermentation (MLF) of wine. This work is the first report on a recombinant  L. plantarum  strain successfully conducting MLF. The malolactic enzyme (MLE) from  Oenococcus oeni  was cloned into the lactobacillal expression vector pSIP409 which is based on the sakacin P operon of  Lactobacillus sakei  and expressed in the host strain  L. plantarum  WCFS1. Both recombinant and wild-type  L. plantarum  strains were tested for MLF using a buffered malic acid solution in absence of glucose. Under the conditions with L-malic acid as the only energy source and in presence of Mn 2+  and NAD + , the recombinant  L. plantarum  and the wild-type strain converted 85% (2.5 g/l) and 51% (1.5 g/l), respectively, of L-malic acid in 3.5 days. Furthermore, the recombinant  L. plantarum  cells converted in a modified wine 15% (0.4 g/l) of initial L-malic acid concentration in 2 days. In conclusion, recombinant  L. plantarum  cells expressing MLE accelerate the malolactic fermentation.

Heterologous expression of Oenococcus oeni malolactic enzyme in Lactobacillus plantarum for improved malolactic fermentation