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Background  B cell chronic lymphocytic leukemia/lymphoma 11 A (BCL11A) is associated with human B cell malignancy initiation. Our previous study has shown that downregulation of  BCL11A  mRNA by small interfering RNA (siRNA) is capable of inducing apoptosis in the SUDHL6 cell line. To further explore the effects of  BCL11A  siRNA on the enhanced cytotoxicity of a chemotherapeutic drug, we investigated the effects of  BCL11A  siRNA combined with vincristine (VCR) on SUDHL6 cell proliferation and apoptosis.    Methods  Chemically synthesized  BCL11A  siRNA was transfected into SUDHL6 cells using the HiPerFect Transfection Reagent in combination with VCR. Cell proliferation was measured by the CCK8 assay. The morphology of apoptotic cells was observed with Hoechst 33258 staining. The rate of cell apoptosis was determined by annexin V-fluorescein isothiocyanate/propidium iodide double staining using fluorescence-activated cell sorting (FACS) analysis.    Results  After  BCL11A  siRNA plus VCR treatment, cell proliferation was significantly decreased in comparison with VCR or  BCL11A  siRNA treatment alone and negative control siRNA plus VCR treatment ( P  <0.05). The apoptotic rate of  BCL11A  siRNA plus VCR treated cells was significantly increased compared with  BCL11A  siRNA and VCR treatment alone and negative control siRNA plus VCR treatment ( P  <0.05).    Conclusions  The combination of  BCL11A  siRNA and VCR increases apoptosis in SUDHL6 cells. Our study implies that  BCL11A  siRNA in combination with VCR may be a useful approach for improving effective treatment for B cell lymphoma.

Combination of BCL11A siRNA with vincristine increases the apoptosis of SUDHL6 cells