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Background  Tea plant, Camellia sinensis (L.) O. Kuntze, a well-known heavy metal hyperaccumulator, possesses a powerful tolerance to heavy metals. The heavy metal stresses lead to reactive oxygen species (ROS) production, and high concentration of ROS is harmful to plants. The glutathione peroxidase gene has positive function to damage induced by ROS. To understand the mechanism of tolerance to deferent stresses in tea plant, a new glutathione peroxidase gene of tea plant was cloned and its expression pattern was analyzed under abiotic and biotic stresses.    Results  A novel cDNA encoding glutathione peroxidase of tea plant (Camellia sinensis) was isolated by rapid amplification of cDNA ends (RACE) method and designated as CsGPX2 (GenBank Accession No. JQ247186). This full-length sequence was 917 nucleotides including a 510 bp open reading frame (ORF), which encoded a polypeptide of 169 amino acids. The deduced amino acid sequence showed high homology with glutathione peroxidases of angiosperms and contained the characteristic conserved motifs of ILAFPCNQF and FTVKD, the highest level of similarity was 85% to a glutathione peroxidase from Ricinus communis (Accession NO. XP_002509790.1). Tissue expression pattern analysis indicated that CsGPX2 expressed similarly in root, stem, leaf and flower of tea plant. The CsGPX2 gene showed strong responses to most abiotic stresses including salinity, heavy metal toxicity, drought, heat, plant hormones, but could not be induced by biotic treatment.    Conclusions  The result suggested that CsGPX2 had potential function in protecting tea plant from peroxidative damage induced by some abiotic stresses.    Electronic supplementary material  The online version of this article (doi:10.1186/1999-3110-55-7) contains supplementary material, which is available to authorized users.

Cloning of a new glutathione peroxidase gene from tea plant (Camellia sinensis) and expression analysis under biotic and abiotic stresses